Regulatory

Part:BBa_K1314000:Design

Designed by: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting   Group: iGEM14_Hong_Kong-CUHK   (2014-10-06)

Ammonium repressible nifH promoter(from Azotobacter vinelandii)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR with 30bp primers. One EcoRI site is removed by overlapping PCR.


Source

A. vinelandii(strain DJ)

References